Analysis Tools · MVA · BUB1B · reanalysis
BUBR1 functional controls: arrest duration
- Archived result
- Executed reanalysis
What it is not. A control for checkpoint competence, not a drug-rescue result. No drug and no N1002K were tested.
Dates, kept apart
- Archived result recorded
- 2026-10-09 (snapshot mva-results-2026-10-17-immunotherapy-audit-v1, data as of 2026-10-17)
- Executions
- Execution time unknown · record packaged 2026-10-24 23:32:43 UTC · this platform
- Execution time unknown · record packaged 2026-10-24 23:32:43 UTC · this platform
- 2026-10-24 20:35:58 UTC · research repository
- 2026-10-22 18:59:19 UTC · research repository
- Repository commit
a434af9- This page rendered
- · a view date, not an execution or publication date
Values read from the files
Read from the verified outputs of execution stackfire-20261024-python312 (CPython 3.12.3, execution time unknown), whose artifacts match the archive exactly or after the documented normalisation shown in the execution record. Nothing is typed into this page.
| Quantity | Value | Unit | Source file |
|---|---|---|---|
| Figure 3D, Wild type: median NEBD-to-exit (40 cells) | 515.01 | minutes | results.json (stackfire-20261024-python312) |
| Figure 3D, V200A/L205A: median NEBD-to-exit (40 cells) | 184.99 | minutes | results.json (stackfire-20261024-python312) |
| Figure 4B, Wild type: median NEBD-to-exit (27 cells) | 650.00 | minutes | results.json (stackfire-20261024-python312) |
| Figure 4B, D137R: median NEBD-to-exit (39 cells) | 60.00 | minutes | results.json (stackfire-20261024-python312) |
| Figure 4F, Wild type: median NEBD-to-exit (40 cells) | 455.00 | minutes | results.json (stackfire-20261024-python312) |
| Figure 4F, C132E: median NEBD-to-exit (40 cells) | 175.01 | minutes | results.json (stackfire-20261024-python312) |
Archived result · October 17 snapshot
Record MVA-CTRL-006 of the frozen evidence snapshot, exactly as published on the case page. Status in the snapshot: Completed; decision: Control only.
- Evidence class
- COMPUTATIONAL / DIRECT-BUBR1
- Model
- Published HeLa BUBR1 depletion/replacement with WT or engineered TPR variants under nocodazole challenge.
- Finding
- Figure 4B source-data medians are 650 minutes for WT and 60 minutes for D137R. A control for checkpoint competence, not a drug-rescue result.
- Next
- Require independent checkpoint and chromosome-segregation readouts in future equal-expression assays.
- Limitations
- D137R is not N1002K; no tested drug or exact patient genotype.
- Cells in the displayed representative replicate are not independent biological replicates.
- Mitotic-arrest duration under spindle challenge is not chromosome-segregation fidelity in unperturbed divisions.
- Provenance in the snapshot
bubr1_checkpoint_kinetics_2026-10-09/results.json· SHA-256d6be3833115ac368fffe185dfea0e42439e4c80e723b88c626ecb9b874073e6cImported an existing analysis artifact and computed its SHA-256. This snapshot build does not rerun the original analysis or validate biological efficacy.
| Row | Median | Note |
|---|---|---|
| Wild type | 650 | 27 cells; representative replicate, not independent biological replicates. |
| D137R | 60 | 39 cells; representative replicate, not independent biological replicates. |
Archive versus execution
The values the frozen snapshot shows, compared with the values read from the executed outputs. Exact where the snapshot stores the full number; otherwise within the decimal places the snapshot itself used.
| Check | Archived | Executed | Tolerance | Result |
|---|---|---|---|---|
| Figure 4B wild type median, chart versus results.json | 650 | 650 | exact | agrees |
| Figure 4B D137R median, chart versus results.json | 60 | 60 | exact | agrees |
Execution records
Each row is a run with a record. “Match” means the output bytes hash to the archived value: deterministic computation reproduced, nothing more.
| Execution / record time | What ran | Environment | Inputs | Artifacts versus archive | Record |
|---|---|---|---|---|---|
| Execution time unknownRecord packaged The original executedAt value was generated by the packaging script, not captured from the pipeline. It is preserved as recordedAt; the actual execution time is unknown.This platform | Execution on this platform (execution time unknown, CPython 3.13.17)Same fresh inputs, CPython 3.13.17 in a new virtual environment: interpreter deliberately outside the pinned version to show which artifact depends on it. | CPython 3.13.17 not the pinned interpreter pandas 2.2.3 · numpy 2.3.5 · scipy 1.17.0 · openpyxl 3.1.5 Isolated virtual environment | 14 / 14 verified by SHA-256Inputs fetched from the public URLs of input_manifest.json over the network, plus the two bundled public references; every byte count and SHA-256 compared with the manifest. |
|
Record (JSON) |
| Execution time unknownRecord packaged The original executedAt value was generated by the packaging script, not captured from the pipeline. It is preserved as recordedAt; the actual execution time is unknown.This platform | Execution on this platform (execution time unknown, CPython 3.12.3)Fresh network downloads of all 12 public inputs on 2026-10-24, each verified against input_manifest.json; system CPython 3.12.3 in a new virtual environment (the pinned 3.12.14 build was not available on this host). | CPython 3.12.3 not the pinned interpreter pandas 2.2.3 · numpy 2.3.5 · scipy 1.17.0 · openpyxl 3.1.5 Isolated virtual environment | 14 / 14 verified by SHA-256Inputs fetched from the public URLs of input_manifest.json over the network, plus the two bundled public references; every byte count and SHA-256 compared with the manifest. |
|
Record (JSON) |
| Research repository | Deterministic replay by the research repository (October 24, 2026)Run in the existing, non-isolated Python 3.12.14 environment before the sensitivity audit. | CPython 3.12.14 pandas 2.2.3 · numpy 2.3.5 · scipy 1.17.0 · openpyxl 3.1.5 Existing environment, not isolated | 14 / 14 verified by SHA-256Previously downloaded public cached bytes, each verified against the unchanged manifest; not a fresh network download. |
|
Record (JSON) At the commit |
| Research repository | Clean reproduction by the research repository (October 22, 2026)Recorded in the research repository. Successful computational reproduction under the stated input boundaries, not biological validation. | CPython 3.12.14 pandas 2.2.3 · numpy 2.3.5 · scipy 1.17.0 · openpyxl 3.1.5 Isolated virtual environment | 12 / 12 verified by SHA-256All 12 remote inputs fetched sequentially into a brand-new isolated virtual environment and verified against input_manifest.json. |
|
Record (JSON) At the commit |
Traceability
- Primary source
- BUBR1 TPR / MCC–APC/C functional controls Figure 4B; supplementary source-data workbook, Sheet1 · DOI 10.1038/s42003-026-10384-9 · PMID 42477419
- Dataset
- Zhang et al. 2026 source data workbook (Sheet1), published with the paper.
- Biological model
- Published HeLa BUBR1 depletion and replacement with WT or engineered TPR variants under nocodazole challenge.
- Samples
- Individual displayed cells per figure panel (3D, 4B, 4F); representative replicate, cells are not independent biological replicates.
- Parameters
- Workbook SHA-256 and panel labels validated before reading
- Per-panel medians of NEBD-to-exit time (minutes)
- Deterministic extraction; no seed
- Script
track2/reproduction/checkpoint/reanalyze.py· SHA-256f74760b9f52747f32352b83b3825ab2960392db41b7ecfb4bd267c5d706b4290· download- Command
python reanalyze.py --input cache/source_data.xlsx --output results.json- Inputs
checkpoint/cache/source_data.xlsx· media.springernature.com · 40.4 KB · SHA-256d793bc309981fefad4a7bc5a8de852054563b0c29b1212d0221519674f9f5f90
Downloads
Provenance manifest (JSON)
Analysis (JSON)
The manifest lists sources, model, samples, parameters, script, commit, inputs, execution records and every file below with its SHA-256. Its generatedAt is when it was written, not an execution date.