BUBR1 recruits PP2A via the B56 family of targeting subunits to promote chromosome congression
Abstract via Crossref
Summary BUBR1 is a mitotic phosphoprotein essential for the maintenance of chromosome stability by promoting chromosome congression and proper kinetochore–microtubule (K-fiber) attachment, but the underlying mechanism(s) has remained elusive. Here we identify BUBR1 as a binding partner of the B56 family of Protein Phosphatase 2A regulatory subunits. The interaction between BUBR1 and the B56 family is required for chromosome congression, since point mutations in BUBR1 that block B56 binding abolish chromosome congression. The BUBR1:B56-PP2A complex opposes Aurora B kinase activity, since loss of the complex can be reverted by inhibiting Aurora B. Importantly, we show that the failure of BUBR1 to recruit B56-PP2A also contributes to the chromosome congression defects found in cells derived from patients with the Mosaic Variegated Aneuploidy (MVA) syndrome. Together, we propose that B56-PP2A is a key mediator of BUBR1's role in chromosome congression and functions by antagonizing Aurora B activity at the kinetochore for establishing stable kinetochore–microtubule attachment at the metaphase plate.
Evidence snapshot note
This reference is cited by the validated MVA · BUB1B evidence snapshot (data as of 2026-10-17) as Xu et al. — BUBR1 recruits PP2A-B56 for chromosome congression.
- Model in the source
- HeLa BUBR1 depletion/reconstitution and BUB1B-mutated MVA fibroblast rescue
- Where in the publication
- Figures 2 and 4; equally expressed B56-binding mutants are functional counterexamples
- Cited by evidence records
- MVA-N1002K-GATE-002
The note describes how the snapshot used the source; it is not a summary of the publication's conclusions.
Identifiers and provenance
- DOI
10.1242/bio.20134051- PMID
23789096- PMCID
PMC3654266- Metadata from
- Crossref, Europe PMC, OpenAlex, fetched
- License (as declared by the source)
- http://creativecommons.org/licenses/by-nc-sa/3.0
- Open access
- Yes
- Added
- from the MVA evidence snapshot
In the library
- Collections
- MVA Hackathon 2026 — BUB1B
- Tags
- None yet
- Keywords from the source
- Chromosome Congression, Bubr1, Aurora B, B56-pp2a